Tissue culture cells - fix with formaldehyde, treat with methanol or acetone. This mAb is also available as a fluorescent conjugate. This highly published monoclonal antibody is validated for use in ELISA, IC, IF, IH, IH(P) & WB. Reliably and specifically detect actin using this Anti-Actin Antibody, clone C4. Mouse monoclonal Ascites fluid, with 0.01% sodium azide. Also, beta- and gamma-actins can readily copolymerize, and the resulting filaments exhibit polymerization and depolymerization rates that vary depending on the ratio of beta- to gamma-actin (Lessard, JL., et al (1988). It has been shown that under calcium-bound conditions, beta-actin exhibits more dynamic behavior than gamma-actin with higher rates of polymerization and depolymerization. Alpha and gamma actins are primarily found in vascular smooth muscle and enteric smooth muscles, respectively. alpha-cardiac and alpha-skeletal actins are expressed in striated cardiac and skeletal muscles, respectively. The other four actin isoforms are typically found in specific adult muscle tissue types. They are nearly identical proteins and differ only in four amino acids at the N-terminal region. Although actins show about 90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues.īeta- and gamma-actins, also known as cytoplasmic actins, are highly conserved in higher animals and are predominantly expressed in non-muscle cells where they control cell structure and motility. At least six different actin types have been reported in mammals. These functions are attributed to their ability to form filaments, which can quickly assemble and disassemble depending upon the needs of the cell. They play critical roles in a wide range of cellular processes, including cell division, cell migration, chromatin remodeling, transcriptional regulation, and vesicle trafficking. 100% Performance Guaranteed Key Spec Table Species ReactivityĪctins are ubiquitous eukaryotic proteins that serve as multi-functional, basic building blocks of cytoskeletal microfilaments.
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